Influence of Different NO3/NH4 on Nitrate and Ammonium Uptake Kinetics of Sugar Beet (Beta vulgaris L.) Seedlings
نویسندگان
چکیده
Solution culture experiments were carried out to study the kinetics at cotyledon stage (11-day-old) and the effect of different NO3/NH4 on NO3 and NH4 uptake at seedling stage (31-day-old) with two cultivars of sugar beet, including Tianyan7 and Tianyan8. NO3 uptake by sugar beet seedlings at cotyledon stage (11-day-old) reached equilibration after 2 hours of adaptation, and NH4 uptake reached equilibration after 6 hours of adaptation. Km values of NH4 uptake by Tianyan7 were lower than Tianyan8, and Vmax values were higher. It was benefit for Tianyan7 to uptake NH4. The kinetics of NO3 and NH4 uptake by the cultivars changed after cultivated in nutrient solution contained different NO3/NH4 for 20 days. NO3 uptake by sugar beet was stimulated by lower concentration of NH4 in the nutrient solution. The NH4 uptake by sugar beet changed complicatedly. Even cultivated in nutrient solution contained pure NH4, the uptake ability of Tianyan7 was higher. Above all, it showed that NH4 uptake of Tianyan7 was higher than Tianyan8, and when NO3/NH4 was 1:4 it reached the highest. This experiment provided a theoretical basis to realize the highly effective ammonium assimilation for sugar beet through the experiment. [Nature and Science. 2004;2(3):70-78].
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